کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1997827 1065620 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Recapitulation of Short RNA-Directed Translational Gene Silencing In Vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Recapitulation of Short RNA-Directed Translational Gene Silencing In Vitro
چکیده انگلیسی

SummarymicroRNAs (miRNAs) are a large class of endogenous short RNAs that repress gene expression. Many miRNAs are conserved throughout evolution, and dysregulation of miRNA pathways has been correlated with an increasing number of human diseases. In animals, miRNAs typically bind to the 3′ untranslated region (3′UTR) of target mRNAs with imperfect sequence complementarity and repress translation. Despite their importance in regulating biological processes in numerous organisms, the mechanisms of miRNA function are largely unknown. Here, we report in vitro reactions for miRNA-directed translational gene silencing. These reactions faithfully recapitulate known in vivo hallmarks of mammalian miRNA function, including a requirement for a 5′ phosphate and perfect complementarity to the mRNA target in the 5′ seed region. Translational gene silencing by miRNAs in vitro requires target mRNAs to possess a 7-methyl G cap and a polyA tail, whereas increasing polyA tail length alone can increase miRNA silencing activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 22, Issue 4, 19 May 2006, Pages 553–560
نویسندگان
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