کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1997926 1065628 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Repositioning of the Reaction Intermediate within the Catalytic Center of the Spliceosome
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Repositioning of the Reaction Intermediate within the Catalytic Center of the Spliceosome
چکیده انگلیسی

SummaryConformational change within the spliceosome is required between the first catalytic step of pre-mRNA splicing, when the branch site attacks the 5′ splice site (SS), and the second step, when the 5′ exon attacks the 3′SS. Little is known, however, about repositioning of the reaction substrates during this transition. Whereas the 5′SS is positioned for the first step by pairing with the invariant U6 snRNA-ACAGAG site, we demonstrate that this pairing interaction must be disrupted to allow transition to the second step. We propose that removal of the branch structure from the catalytic center is in competition with binding of the 3′SS substrate for the second step. Changes in the relative occupancy of first and second step substrates at the catalytic center alter efficiency of the two steps of splicing, allowing use of suboptimal intron sequences and thereby altering substrate selectivity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 21, Issue 4, 17 February 2006, Pages 543–553
نویسندگان
, , ,