کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1997945 1065630 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural Basis for Processivity and Single-Strand Specificity of RNase II
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Structural Basis for Processivity and Single-Strand Specificity of RNase II
چکیده انگلیسی

SummaryRNase II is a member of the widely distributed RNR family of exoribonucleases, which are highly processive 3′→5′ hydrolytic enzymes that play an important role in mRNA decay. Here, we report the crystal structure of E. coli RNase II, which reveals an architecture reminiscent of the RNA exosome. Three RNA-binding domains come together to form a clamp-like assembly, which can only accommodate single-stranded RNA. This leads into a narrow, basic channel that ends at the putative catalytic center that is completely enclosed within the body of the protein. The putative path for RNA agrees well with biochemical data indicating that a 3′ single strand overhang of 7–10 nt is necessary for binding and hydrolysis by RNase II. The presence of the clamp and the narrow channel provides an explanation for the processivity of RNase II and for why its action is limited to single-stranded RNA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 24, Issue 1, 6 October 2006, Pages 149–156
نویسندگان
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