کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1998033 1065640 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
S. cerevisiae Tel1p and Mre11p Are Required for Normal Levels of Est1p and Est2p Telomere Association
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
S. cerevisiae Tel1p and Mre11p Are Required for Normal Levels of Est1p and Est2p Telomere Association
چکیده انگلیسی

SummaryIn diverse organisms, the Mre11 complex and phosphoinositide 3-kinase-related kinases (PIKKs), such as Tel1p and Mec1p from S. cerevisiae, are key mediators of DNA repair and DNA damage checkpoints that also function at telomeres. Here, we use chromatin immunoprecipitation (ChIP) to determine if Mre11p, Tel1p, or Mec1p affects telomere maintenance by promoting recruitment of telomerase subunits to S. cerevisiae telomeres. We find that recruitment of Est2p, the catalytic subunit of telomerase, and Est1p, a telomerase accessory protein, was severely reduced in mre11Δ and tel1Δ cells. In contrast, the levels of Est2p and Est1p binding in late S/G2 phase, the period in the cell cycle when yeast telomerase lengthens telomeres, were indistinguishable in wild-type (WT) and mec1Δ cells. These data argue that Mre11p and Tel1p affect telomere length by promoting telomerase recruitment to telomeres, whereas Mec1p has only a minor role in telomerase recruitment in a TEL1 cell.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 24, Issue 4, 17 November 2006, Pages 603–610
نویسندگان
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