کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1998046 1065641 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Engineered Hybrid Dimers: Tracking the Activation Pathway of Caspase-7
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Engineered Hybrid Dimers: Tracking the Activation Pathway of Caspase-7
چکیده انگلیسی

SummaryCaspase-7 is an obligate dimer of catalytic domains, with generation of activity requiring limited proteolysis within a region that separates the large and small chains of each domain. Using hybrid dimers we distinguish the relative contribution of each domain to catalysis by the whole molecule. We demonstrate that the zymogen arises from direct dimerization and not domain swapping. In contrast to previous conclusions, we show that only one of the catalytic domains must be proteolyzed to enable activation. The processed domain of this singly cleaved zymogen has the same catalytic activity as a domain of fully active caspase-7. A transient intermediate of singly cleaved dimeric caspase-7 can be found in a cell-free model of apoptosis induction. However, we see no evidence for an analogous intermediate of the related executioner caspase-3. Our study demonstrates the efficiency by which the executioner caspases are activated in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 23, Issue 4, 18 August 2006, Pages 523–533
نویسندگان
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