کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1998139 1065650 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A Uniform Response to Mismatches in Codon-Anticodon Complexes Ensures Ribosomal Fidelity
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A Uniform Response to Mismatches in Codon-Anticodon Complexes Ensures Ribosomal Fidelity
چکیده انگلیسی

SummaryRibosomes take an active part in aminoacyl-tRNA selection by distinguishing correct and incorrect codon-anticodon pairs. Correct codon-anticodon complexes are recognized by a network of ribosome contacts that are specific for each position of the codon-anticodon duplex and involve A-minor RNA interactions. Here, we show by kinetic analysis that single mismatches at any position of the codon-anticodon complex result in slower forward reactions and a uniformly 1000-fold faster dissociation of the tRNA from the ribosome. This suggests that high-fidelity tRNA selection is achieved by a conformational switch of the decoding site between accepting and rejecting modes, regardless of the thermodynamic stability of the respective codon-anticodon complexes or their docking partners at the decoding site. The forward reactions on mismatched codons were particularly sensitive to the disruption of the A-minor interactions with 16S rRNA and determined the variations in the misreading efficiency of near-cognate codons.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 21, Issue 3, 3 February 2006, Pages 369–377
نویسندگان
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