کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1998166 1065652 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Making and Breaking Nucleic Acids: Two-Mg2+-Ion Catalysis and Substrate Specificity
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Making and Breaking Nucleic Acids: Two-Mg2+-Ion Catalysis and Substrate Specificity
چکیده انگلیسی

SummaryDNA and a large proportion of RNA are antiparallel duplexes composed of an unvarying phosphosugar backbone surrounding uniformly stacked and highly similar base pairs. How do the myriad of enzymes (including ribozymes) that perform catalysis on nucleic acids achieve exquisite structure or sequence specificity? In all DNA and RNA polymerases and many nucleases and transposases, two Mg2+ ions are jointly coordinated by the nucleic acid substrate and catalytic residues of the enzyme. Based on the exquisite sensitivity of Mg2+ ions to the ligand geometry and electrostatic environment, we propose that two-metal-ion catalysis greatly enhances substrate recognition and catalytic specificity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 22, Issue 1, 7 April 2006, Pages 5–13
نویسندگان
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