کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2000519 | 1541615 | 2015 | 8 صفحه PDF | دانلود رایگان |
• Decomposition of IPA/NO provided a new route to ONOO− in millimolar concentrations.
• Formation of ONOO− from H2O2 and NO2− showed a preparation-dependent variability.
• HNO autoxidation results in a unique intermediate from synthetic ONOO−.
Donors of nitroxyl (HNO) exhibit pharmacological properties that are potentially favorable for treatment of a variety of diseases. To fully evaluate the pharmacological utility of HNO, it is therefore important to understand its chemistry, particularly involvement in deleterious biological reactions. Of particular note is the cytotoxic species formed from HNO autoxidation that is capable of inducing double strand DNA breaks. The identity of this species remains elusive, but a conceivable product is peroxynitrous acid. However, chemical comparison studies have demonstrated that HNO autoxidation leads to a unique reactive nitrogen oxide species to that of synthetic peroxynitrite. Here, we extend the analysis to include a new preparation of peroxynitrite formed via autoxidation of nitroxyl anion (NO−). Both peroxynitrite preparations exhibited similar chemical profiles, although autoxidation of NO− provided a more reliable sample of peroxynitrite. Furthermore, the observed dissimilarities to the HNO donor Angeli's salt substantiate that HNO autoxidation produces a unique intermediate from peroxynitrite.
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Journal: Nitric Oxide - Volume 44, 30 January 2015, Pages 39–46