کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2001275 1066027 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nitrite and nitrate reduction by molybdenum centers of the nitrate reductase type: Computational predictions on the catalytic mechanism
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Nitrite and nitrate reduction by molybdenum centers of the nitrate reductase type: Computational predictions on the catalytic mechanism
چکیده انگلیسی

Nitrate reductases (NRs) are enzymes that catalyze reduction of nitrate to nitrite using a molybdenum cofactor. In an alternative reaction, plant NRs have also been shown to catalyze reduction of nitrite to nitric oxide, and this appears to be a major source of nitric oxide synthesis in plants, although other pathways have also been shown. Here, density functional theory (DFT) results are shown, indicating that although nitrate is thermodynamically the preferred substrate for the NR active site, both nitrite and nitrate are easily reduced to nitrite and NO, respectively. These mechanisms require a Mo(IV) state. Additionally, in the case of the nitrite, linkage isomerism is at work and controlled by the metal oxidation state, and reduction is, unlike in the nitrate case, dependent on protonation. The data may be relevant to other molybdenum enzymes with similar active sites, such as xanthine oxidase.


► DFT results are shown on nitrate and nitrite reduction in nitrite reductases.
► Nitrate is thermodynamically the preferred substrate.
► Nitrite and nitrate are both easily reduced to nitrite and NO, respectively.
► These reductions require a Mo(IV) state.
► In the case of the nitrite, linkage isomerism and proton dependence are predicted.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nitric Oxide - Volume 26, Issue 1, 1 January 2012, Pages 27–31
نویسندگان
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