کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2001715 1066057 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Selective fluorescent labeling of S-nitrosothiols (S-FLOS): A novel method for studying S-nitrosation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Selective fluorescent labeling of S-nitrosothiols (S-FLOS): A novel method for studying S-nitrosation
چکیده انگلیسی

Protein S-nitrosation is a reversible post-translation modification critical for redox-sensitive cell signaling that is typically studied using the Biotin Switch method. This method and subsequent modifications usually require avidin binding or Western blot analysis to detect biotin labeled proteins. We describe here a modification of the Biotin Switch assay that eliminates the need for Western blot or avidin enrichment protocols and allows direct comparison of the S-nitrosation state proteins from two different samples in the same gel lane or on the same 2D gel. This S-FLOS method offers detection, identification and quantification of S-nitrosated proteins, with the potential for site-specific identification of nitrosation events.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nitric Oxide - Volume 19, Issue 3, November 2008, Pages 295–302
نویسندگان
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