کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
20140 43159 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A rapid method for simultaneous evaluation of free light chain content and aggregate content in culture media of Chinese hamster ovary cells expressing monoclonal antibodies for cell line screening
ترجمه فارسی عنوان
یک روش سریع برای بررسی همزمان محتوای زنجیره آزاد و محتوای جامد در محیط کشت سلول های تخمدان هامستر چین، که آنتیبادیهای منوکلونال را برای غربالگری سلول
کلمات کلیدی
آنتی بادی درمانی، اندازه گیری کروماتوگرافی حذف، گونه های کم مولکولی، گونه های با وزن مولکولی بالا
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• A rapid, simple, reliable method for evaluating cells expressing mAb is reported.
• This method determines the contents of light chain in culture medium.
• This method determines the aggregate content in medium without prior purification.

The goal of developing a monoclonal antibody (mAb) production process is high productivity and high quality. Because the productivity and quality of mAbs depend on cell line properties, the selection of cell lines suitable for large-scale production is an important stage in process development for mAb production. The light chain (LC) is important for antibody folding and assembly in the endoplasmic reticulum; cell lines that secrete a large amount of LCs in the medium secrete high-quality antibodies with high productivity. LC contents in culture media have been estimated by western blotting, reverse-phase high-performance liquid chromatography, and enzyme-linked immunosorbent assay. However, these analyses require fine tuning of experimental conditions for each antibody analyzed. Here we report a rapid and simple high-sensitivity size-exclusion chromatography (HS-SEC) method to evaluate the contents of low-molecular weight species (LMWS, mainly consisting of LC monomers and dimers) and high-molecular weight species (HMWS, aggregates) in the media for cell line screening. Because LMWS and HMWS are important indicators of productivity and quality, respectively, for cell line screening, HS-SEC will be useful in the first step of cell line selection needed for large-scale production.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 4, April 2016, Pages 464–470
نویسندگان
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