کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2014289 | 1067151 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Participation of the GABA/benzodiazepine receptor and the NO-cyclicGMP pathway in the “antinociceptive-like effects” of diazepam
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The mechanism of action underlying the “analgesic activity” of diazepam remains unclear. In this study, the possible participation of the GABA/benzodiazepine receptor and the nitric oxide-cyclic GMP (NO-cGMP) pathway was assessed utilizing the pain-induced functional impairment model in the rat (PIFIR). Nociception was induced by an intra-articular injection of 15% uric acid. Diazepam (1 and 2Â mg/kg, i.p.) reversed the dysfunction induced by uric acid. Flumazenil (10Â mg/kg, i.p.), a GABA/benzodiazepine receptor antagonist, abolished the “antinociceptive-like effect” of diazepam (at 2Â mg/kg). The “antinociceptive-like effect” of diazepam (at 2Â mg/kg) was antagonized by the non-selective nitric oxide synthase (NOS) inhibitor, NÏ-l-nitro-arginine methyl ester hydrochloride (l-NAME, 5Â mg/kg, s.c.) (but not by its inactive isomer), and by the selective neuronal NOS inhibitor, 7-nitroindazole (7-NI, 1Â mg/kg, i.p). While, the NO precursor, l-arginine (125Â mg/kg, s.c.), but not d-arginine (125Â mg/kg, s.c.), increased the “antinociceptive-like effect” of a non-effective dose of diazepam (1Â mg/kg). Methylene blue (10Â mg/kg, i.p.), a guanylate cyclase inhibitor, also prevented the “antinociceptive-like effect” of diazepam (at 2Â mg/kg). These results suggest that the GABA/benzodiazepine receptor and the NO-cGMP pathway participate in the “antinociceptive-like effect” of diazepam.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pharmacology Biochemistry and Behavior - Volume 91, Issue 1, November 2008, Pages 128-133
Journal: Pharmacology Biochemistry and Behavior - Volume 91, Issue 1, November 2008, Pages 128-133
نویسندگان
Guadalupe Jiménez-Velázquez, Francisco Javier López-Muñoz, Alonso Fernández-Guasti,