کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2014736 1541913 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ROP6 is involved in root hair deformation induced by Nod factors in Lotus japonicus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
ROP6 is involved in root hair deformation induced by Nod factors in Lotus japonicus
چکیده انگلیسی


• We identify ten LjROP GTPases including LjROP6 in L. japonicus.
• LjROP6 GTPase binds NFR5 in a GTP-dependent manner.
• Overexpression of LjROP6-CA exhibited more root hair deformation, resulting increment of infection thread and nodule number.
• LjROP6 is involved in root hair deformation responses induced by NFs in early stage of symbiotic interaction in L. japonicus.

Roots of leguminous plants perceive Nod factor signals, and then root hair deformation responses such as swelling and curling are activated. However, very little is known about the molecular mechanisms of such root hair deformation. We have previously shown that LjROP6, a member of the Rho family of small GTPases, was identified as an NFR5 (Nod Factor Receptor 5)-interacting protein and participated in symbiotic nodulation in Lotus japonicus. In this study, we identified ten LjROP GTPases including LjROP6, and they were distributed into groups II, III, IV but not group I by phylogenetic analysis. The expression profiles of ten LjROP genes during nodulation were examined. LjROP6 belonged to group IV and interacted with NFR5 in a GTP-dependent manner. Overexpression of either wild-type ROP6 or a constitutively active mutant (ROP6-CA) generated root hair tip growth depolarization, while overexpression of a dominant negative mutant (ROP6-DN) exhibited normal root hair growth. After inoculating with Mesorhizobium loti or adding Nod factors to hairy roots, overexpression of ROP6 and ROP6-CA exhibited extensive root hair deformation, while overexpression of ROP6-DN inhibited root hair deformation. The infection event and nodule number were increased in ROP6 and ROP6-CA overexpressing transgenic plants; but decreased in ROP6-DN overexpressing transgenic plants. These studies provide strong evidence that ROP6 GTPase, which binds NFR5 in a GTP-dependent manner, is involved in root hair development as well as root hair deformation responses induced by NFs in the early stage of symbiotic interaction in L. japonicus.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 108, November 2016, Pages 488–498
نویسندگان
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