کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2015011 1541964 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Heat exposure alters the expression of SOD, POD, APX and CAT isozymes and mitigates low cadmium toxicity in seedlings of sensitive and tolerant rice cultivars
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Heat exposure alters the expression of SOD, POD, APX and CAT isozymes and mitigates low cadmium toxicity in seedlings of sensitive and tolerant rice cultivars
چکیده انگلیسی

A 0–500 μM Cd2+ alone and/or heat stress in rice cv. DR-92 (sensitive) and cv. Bh-1 (tolerant), altered the banding patterns of SOD, CAT, POD and APX enzymes in roots/shoots. In controls, six/seven activity bands for POD in roots/shoots were observed. The band intensities of some decreased under combination of Cd2+ + heat stress. Six SOD isoforms in shoots of cv. Bh-1 and three in cv. DR-92 appeared. In sensitive cv. DR-92 a trinuclear Cu/Zn/Mn-SOD 1 isozyme was upregulated in shoots under Cd/HS/Cd + HS treatments whereas a suppression in the same was noticed in roots. Under Cd alone the Cu/Zn/Mn-SOD 2 was strongly induced in roots which was otherwise absent in all HS treatments. POD R7 band was absent in HS alone but was induced under Cd2+ + HS treatments in rice cv. DR-92. In tolerant cv. Bh-1, isozyme Mn-SOD 3 was induced under Cd alone but was absent under HS. Cu/Zn/Mn-SOD 2 and Cu/Zn/Mn-SOD 3 were strongly induced in roots and shoots under low or moderate Cd2+ + HS treatments. APX R4/CATR2 isozymes which were absent under Cd2+ alone or HS alone were induced under combination of Cd2+ + HS indicating de novo synthesis of enzyme proteins under combined stressors. Decreased band intensities under Cd2+ + HS suggest a cross-talk between response pathways of Cd2+ and heat stress in rice. Results suggest Cd2+ specific, heat-specific, tissue specific and differential expression of SOD/POD/APX/CAT and that Mn-SOD 3/APXR4/CATR2 seem to form important components of antioxidant defense in rice roots under combination of Cd2+ + HS which helps to mitigate the effect of low Cd2+ toxicity in tolerant rice cv. Bh-1.


► HS + Cd2+ induce differential expression of SOD, CAT, and APX isozymes.
► SOD/APX isozymes and POD activity groups form important component of plant defense under Cd2+ + HS.
► Existence of trinuclear SOD isoforms in rice regulated by Cd2+ + HS is indicated.
► Both Cd2+ specific and heat-specific responses to isoform banding patterns were noted.
► A cross-protection of low Cd2+ toxicity by heat stress in rice is indicated.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 57, August 2012, Pages 106–113
نویسندگان
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