Application of the split root technique to study iron uptake in cucumber plants

► The split root technique was used to study the response to Fe deficiency in cucumber roots. ► Important morphological changes at root level after split root application were observed. ► Enzyme activity of FRO1, H+-ATPase and PEPC and immunolocalization of H+-ATPase and PEPC were determined. ► Different coregulation among FRO1, IRT1, HA1 and PEPC1 genes was found.