کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2015524 | 1541916 | 2016 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genome-wide identification and expression analysis of the metacaspase gene family in Hevea brasiliensis
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کلمات کلیدی
TSATapping panel drynessPCDqRT-PCRORFTPDHevea brasiliensisLSDEthephon - اتفونEST - استAbiotic stress - استرس بی روحExpressed Sequence Tag - تکرار اظهار نظرopen reading frame - قاب خواندن بازMetacaspase - متاکاسپازMethyl jasmonate - متیل جاسموناتProgrammed cell death - مرگ برنامهریزی شده یاختهTranscriptome shotgun assembly - مونتاژ تفنگ ساچمه ایMeJA - میاquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Metacaspases, a family of cysteine proteases, have been suggested to play important roles in programmed cell death (PCD) during plant development and stress responses. To date, no systematic characterization of this gene family has been reported in rubber tree (Hevea brasiliensis). In the present study, nine metacaspase genes, designated as HbMC1 to HbMC9, were identified from whole-genome sequence of rubber tree. Multiple sequence alignment and phylogenetic analyses suggested that these genes were divided into two types: type I (HbMC1-HBMC7) and type II (HbMC8 and HbMC9). Gene structure analysis demonstrated that type I and type II HbMCs separately contained four and two introns, indicating the conserved exon-intron organization of HbMCs. Quantitative real-time PCR analysis revealed that HbMCs showed distinct expression patterns in different tissues, suggesting the functional diversity of HbMCs in various tissues during development. Most of the HbMCs were regulated by drought, cold, and salt stress, implying their possible functions in regulating abiotic stress-induced cell death. Of the nine HbMCs, HbMC1, HbMC2, HbMC5, and HbMC8 displayed a significantly higher relative transcript accumulation in barks of tapping panel dryness (TPD) trees compared with healthy trees. In addition, the four genes were up-regulated by ethephon (ET) and methyl jasmonate (MeJA), indicating their potential involvement in TPD resulting from ET- or JA-induced PCD. In summary, this work provides valuable information for further functional characterization of HbMC genes in rubber tree.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 105, August 2016, Pages 90-101
Journal: Plant Physiology and Biochemistry - Volume 105, August 2016, Pages 90-101
نویسندگان
Hui Liu, Zhi Deng, Jiangshu Chen, Sen Wang, Lili Hao, Dejun Li,