کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2016126 1541961 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hydrophobic proteins secreted into the apoplast may contribute to resistance against Phytophthora infestans in potato
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Hydrophobic proteins secreted into the apoplast may contribute to resistance against Phytophthora infestans in potato
چکیده انگلیسی

During plant–pathogen interaction, oomycetes secrete effectors into the plant apoplast where they interact with host resistance proteins, which are accumulated after wounding or infection. Previous studies showed that the expression profile of pathogenesis related proteins is proportional to the resistance of different cultivars toward Phytophthora infestans infection. The aim of this work was to analyze the expression pattern of apoplastic hydrophobic proteins (AHPs), after 24 h of wounding or infection, in tubers from two potato cultivars with different resistance to P. infestans, Spunta (susceptible) and Innovator (resistant). Intercellular washing fluid (IWF) was extracted from tubers and chromatographed into a PepRPC™ HR5-5 column in FPLC eluted with a linear gradient of 75% acetonitrile. Then, AHPs were analyzed by SDS-PAGE and identified by MALDI-TOF-MS. Innovator cv. showed a higher basal AHP content compared to Spunta cv. In the latter, infection induced accumulation of patatins and protease inhibitors (PIs), whereas in Innovator cv. no changes in PIs accumulation were observed. In response to P. infestans infection, lipoxygenase, enolase, annexin p34 and glutarredoxin/cyclophilin were accumulated in both cultivars. These results suggest that the AHPs content may be related to the protection against the oomycete and with the degree of potato resistance to pathogens. Additionally, a considerable number of the proteins putatively identified lacked the signal peptide and, being SecretomeP positive, suggest unconventional protein secretion.


► Constitutive and inducible levels of AHPs are correlated with the field resistance degree of potato to Pi.
► Unconventional protein secretion pathway is present during potato-Pi interaction.
► AHPs identified in potato tuber IWF are majority identified as protease inhibitors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 60, November 2012, Pages 59–66
نویسندگان
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