|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|2016236||1541944||2014||6 صفحه PDF||سفارش دهید||دانلود رایگان|
• A polyphenol-rich extract from grapes induced chemoperception in tobacco suspension cells.
• GME induced modification of plasma membrane properties.
• GME elicited defence genes in tobacco cells.
• GME-induced cell death was mediated by proteases and de novo protein synthesis.
• This study evidences the ability of GME to induce hypersensitive response.
Grape marc extract (GME) showed elicitor activity on suspension-cultured cells of tobacco. The BY-2 cells reacted to GME (0.25% and 0.125%) with a long-sustained pH rise in their growth medium. Using EGTA or LaCl3, we showed that extracellular alkalinization depended on Ca2+ mobilization. The tobacco BY-2 cells challenged with GME promoted cell death and the upregulation of defence-related genes such as PR3, PAL and CCoAOMT. Cell death rate was quantified using an experimental calibrated Evans Blue assay. The GME-induced cell death was dose-dependent and occurred in 24 h. Longer exposure increased the extent of tobacco cell death. To investigate a potential hypersensitive reaction, we tested the effect of various inhibitors of protein synthesis (cycloheximide) and proteases (aprotinin, pepstatin and E-64) on GME-induced cell death. All these chemicals reduced GME-induced cell death rate in 30 min. Overall, our findings indicate that GME elicits early perception events, defence reactions and cell death requiring protein synthesis and proteases.
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Journal: Plant Physiology and Biochemistry - Volume 77, April 2014, Pages 84–89