کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2016469 | 1067662 | 2010 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ralstonia solanacearum induces soluble amine-oxidase activity in Solanum torvum stem calli
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کلمات کلیدی
PHAPAOPHpRalstonia solanacearumVmaxSSAOSolanum torvumCalliTPQBSA - BSAbovine serum albumine - آلبومین سرم گاوamine oxidase - آمین اکسیدازSemicarbazide sensitive amine oxidase - آمین اکسیداز مقاوم به سمی کربراازیدEDTA - اتیلن دی آمین تترا استیک اسید DAO - دائوDiamine oxidase - دیامین اکسیدازMichaelis constant - مایکلز ثابت استHypersensitive response - پاسخ حساسیتPolyamines - پلی آمینpolyamine oxidase - پلیامین اکسیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Solanum torvum is reported to carry resistance to bacterial wilt caused by Ralstonia solanacearum. So, this wild species is used as rootskock for eggplants or tomatoes in naturally infected soil. This study aimed to investigate the involvement of the polyamine metabolism pathway in the resistance mechanisms of this species. Calli induced from Solanum torvum stem explants were inoculated with the bacteria under partial vacuum. All calli showed a hypersensitive response after infiltration. Furthermore, amine oxidase activity with aldehyde and H2O2 production was detected in soluble protein extracts of calli infiltrated by the bacteria. Due to its preferential affinity for aliphatic amines, this enzyme was supposed to have amine oxidase-like (AO-like) activity. Moreover, the length of aliphatic chain cycle altered the oxidative deamination kinetics of potential substrates. The AO-like catalytic activity was significantly inhibited by chelator agents such as ethylene-diamine-tretraacetic (EDTA), and also by semi-carbazide as aminoguanidine. These results suggested that (i) the prosthetic group of the AO-like enzyme could be a tyrosine-derived 6-hydroxytopaquinone structure, copper containing; (ii) this enzyme could be a semi-carbazide sensitive amine oxidase (SSAO).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 48, Issue 9, September 2010, Pages 787-796
Journal: Plant Physiology and Biochemistry - Volume 48, Issue 9, September 2010, Pages 787-796
نویسندگان
Marcel Aribaud, Sylvaine Jégo, Emmanuel Wicker, Isabelle Fock,