کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2016911 | 1542039 | 2016 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tissue-specific expression of Arabidopsis NPR1 gene in rice for sheath blight resistance without compromising phenotypic cost
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کلمات کلیدی
DPIPDISARPDABDLMurashige and Skoog - Murashige و SkoogTransgenic rice - برنج ترانس ژنیکdays post inoculation - روز بعد از تلقیحpotato dextrose agar - سیب زمینی دگزستروز آگارsystemic acquired resistance - سیستمیک به دست آورد مقاومتYield penalty - مجازاتDisease management - مدیریت بیماریPathogenesis-related genes - ژن های مربوط به پاتوژنز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Rice sheath blight disease, caused by the fungus Rhizoctonia solani, is considered the second most important disease of rice after blast. NPR1 (non expressor of PR1) is the central regulator of systemic acquired resistance (SAR) conferring broad spectrum resistance to various pathogens. Previous reports have indicated that constitutive expression of the Arabidopsis thaliana NPR1 (AtNPR1) gene results in disease resistance in rice but has a negative impact on growth and agronomic traits. Here, we report that green tissue-specific expression of AtNPR1 in rice confers resistance to the sheath blight pathogen, with no concomitant abnormalities in plant growth and yield parameters. Elevated levels of NPR1 activated the defence pathway in the transgenic plants by inducing expression of endogenous genes such as PR1b, RC24, and PR10A. Enhanced sheath blight resistance of the transgenic plants was evaluated using three different bioassay systems. A partially isolated toxin from R. solani was used in the bioassays to measure the resistance level. Studies of the phenotype and yield showed that the transgenic plants did not exhibit any kind of phenotypic imbalances. Our results demonstrate that green tissue-specific expression of AtNPR1 is an effective strategy for controlling the sheath blight pathogen. The present work in rice can be extended to other crop plants severely damaged by the pathogen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 250, September 2016, Pages 105-114
Journal: Plant Science - Volume 250, September 2016, Pages 105-114
نویسندگان
Kutubuddin Ali Molla, Subhasis Karmakar, Palas Kumar Chanda, Sailendra Nath Sarkar, Swapan Kumar Datta, Karabi Datta,