کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2016971 | 1542052 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
PHYTOCHROME-INTERACTING FACTOR 5 (PIF5) positively regulates dark-induced senescence and chlorophyll degradation in Arabidopsis
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کلمات کلیدی
PIFNOLreal-time quantitative reverse transcription PCRCLHSAGPAOSGRTBARSqRT-PCRFLuc - FLUCArabidopsis thaliana - آرابیدوپسیس تالیاناElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزchromatin immunoprecipitation - ایمن سازی کروماتینChlorophyll degradation - تخریب کلروفیلrenilla luciferase - رگیلا لوسیفرازStay green - سبز بمانEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکFirefly luciferase - لوسیفراز فیرفیلیthiobarbituric acid reactive substance - واکنش پذیر اسید تیوباربیتوریکCHiP - چیپSenescence-associated gene - ژن وابسته به سن زبانیChlorophyllase - کلروفیل اسید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Darkness is a known environmental factor that induces plant senescence. Here, PHYTOCHROME-INTERACTING FACTORS (PIFs), several bHLH transcription factors involved in plant skotomorphogenesis, were examined for their roles in the regulation of dark-induced senescence and chlorophyll breakdown in Arabidopsis thaliana. After light-grown seedlings were transferred to darkness, green leaves turned yellow, and chlorophyll contents decreased, but membrane lipid peroxidation and cell death increased in wild-type Col-0. These responses were enhanced in overexpression line PIF5OX but decreased in mutant pif5-3. Darkness significantly induced expression of several genes involved in chlorophyll breakdown, including SGR, NYC1, NOL, and PAO, as well as genes encoding for transcription factors that have been shown to be required for dark-induced senescence, including WRKY22, NAP, EIN3, EIL1, and ORE1. These effects on gene expression were also enhanced in PIF5OX but decreased in pif5-3 relative to Col-0. Further analyses using ChIP-qPCR, EMSA, and protoplast transient assays indicated that PIF5 binds to the G-box motifs in the promoters of SGR, NYC1, and ORE1 genes and stimulate their expression. Collectively, our data indicate that PIF5 is a key factor that positively regulates dark-induced senescence upstream of ORE1 and regulates chlorophyll breakdown upstream of SGR and NYC1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 237, August 2015, Pages 57-68
Journal: Plant Science - Volume 237, August 2015, Pages 57-68
نویسندگان
Yongqiang Zhang, Zhongjuan Liu, Yadi Chen, Jun-Xian He, Yurong Bi,