کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2017091 1542067 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Generation and characterization of a tomato DCL3-silencing mutant
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Generation and characterization of a tomato DCL3-silencing mutant
چکیده انگلیسی


• Cloning the tomato DCL3 homolog and its silencing by artificial miRNA.
• SlDCL3 silencing decreases 24 nt small RNA numbers and diversity.
• SlDCL3 silencing increases 21–22 nt small RNA numbers and diversity.
• SlDCL2b and SlDCL4 are upregulated upon SlDCL3 silencing.
• Identification of novel SlDCL3-dependent 24 nt tomato long miRNAs.

DICER-like 3 (DCL3) is a major player in heterochromatic 24-nucleotide (nt) small RNA (sRNA) and long microRNA (lmiRNA) biogenesis, and higher plant DCL3 mutants have been characterized from Arabidopsis thaliana and rice. Here, a tomato DCL3 (SlDCL3) mutant was generated through the use of trans-activated artificial miRNA and characterized. Constitutive trans-activation knocked down SlDCL3 levels by ∼64%, resulting in dramatically decreased 24-nt sRNA levels and a significant increase in 21- and 22-nt sRNAs. The latter was correlated with specific upregulation of SlDCL4 and SlDCL2b, which function in the biogenesis of 21- and 22-nt sRNAs, respectively. Moreover, at the majority of sRNA-generating genomic loci, an almost complete overlap between small RNA signatures of control and silenced seedlings was observed, suggesting that the reductions in 24-nt sRNAs at these loci were compensated for by biogenesis of 21- and 22-nt sRNAs from the same double-stranded RNA substrates. In addition, bioinformatic analysis and reduced expression in SlDCL3-silenced seedlings identified four novel tomato lmiRNAs, two of which were found to be developmentally regulated. Taken together, these results establish the requirement of SlDCL3 for the biogenesis of 24-nt sRNAs and lmiRNAs in tomato and suggest SlDCL4 and SlDCL2b as surrogates for SlDCL3.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volumes 221–222, May 2014, Pages 81–89
نویسندگان
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