Single nucleotide polymorphism isolated from a novel EST dataset in garden asparagus (Asparagus officinalis L.)

► Pyro-sequencing has been used to sequence transcriptome from garden asparagus. ► A pipeline has been used to predict SNPs and SSR sequences in asparagus. ► Gene Ontology annotation assignments for all uniscripts/genes functions has been adopted. ► Over 1800 putative SNPs and 1000 SSRs were detected from the assembled uniscripts. ► 144 SNPs together with 60 SSRs were utilized for mapping in a segregant population.