Physiological and biochemical changes of CBF3 transgenic oat in response to salinity stress

Salinity is a major abiotic constraint affecting oat productivity. Several physiological and biochemical traits have been found to be related to yield maintenance under salinity. The impact of introducing the Arabidopsis CBF3 gene controlled by the rd29A stress-inducible promoter in T2 transgenic oat on salinity tolerance and associated physiological changes were studied. Compared with the non-transgenic control, transgenic T2 plants exhibited greater growth and showed significant maintenance of leaf area, relative water content, chlorophyll content, photosynthetic and transpiration rates as well as increased levels of proline and soluble sugars under high salt stress. These physiological changes delayed leaf-wilting symptoms, increased tolerance and reduced yield loss. At a salinity stress level of 100 mM, the CBF3-overexpressing transgenic oat showed a yield loss of 4–11% compared with >56% for the non-transgenic control. These results demonstrate that stress-inducible over-expression of CBF3 may have the potential to enhance abiotic stress tolerance in oat.

► The physiological response associated with improved salinity tolerance in CBF3 T2 transgenic oat was tested.
► Transgenic plants showed a significant maintenance of physiological and biochemical traits as well as yield under salinity.
► This confirms the role of the Arabidopsis CBF3 as a key regulatory gene to enhance salinity tolerance.