کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2017496 1067800 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Double-filter identification of vascular-expressed genes using Arabidopsis plants with vascular hypertrophy and hypotrophy
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Double-filter identification of vascular-expressed genes using Arabidopsis plants with vascular hypertrophy and hypotrophy
چکیده انگلیسی

Genes expressed in vascular tissues have been identified by several strategies, usually with a focus on mature vascular cells. In this study, we explored the possibility of using two opposite types of altered tissue compositions in combination with a double-filter selection to identify genes with a high probability of vascular expression in early organ primordia. Specifically, we generated full-transcriptome microarray profiles of plants with (a) genetically strongly reduced and (b) pharmacologically vastly increased vascular tissues and identified a reproducible cohort of 158 transcripts that fulfilled the dual requirement of being underrepresented in (a) and overrepresented in (b). In order to assess the predictive value of our identification scheme for vascular gene expression, we determined the expression patterns of genes in two unbiased subsamples. First, we assessed the expression patterns of all twenty annotated transcription factor genes from the cohort of 158 genes and found that seventeen of the twenty genes were preferentially expressed in leaf vascular cells. Remarkably, fifteen of these seventeen vascular genes were clearly expressed already very early in leaf vein development. Twelve genes with published leaf expression patterns served as a second subsample to monitor the representation of vascular genes in our cohort. Of those twelve genes, eleven were preferentially expressed in leaf vascular tissues.Based on these results we propose that our compendium of 158 genes represents a sample that is highly enriched for genes expressed in vascular tissues and that our approach is particularly suited to detect genes expressed in vascular cell lineages at early stages of their inception.


► We alter leaf vascular content to identify vascular regulators.
► We compare plants with genetically reduced and pharmacologically increased vascular tissues.
► 158 transcripts were differentially expressed.
► We asses expression pattern of all transcription factors and published genes.
► We predict that 90% of out cohort of genes has vascular expression.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 181, Issue 2, August 2011, Pages 96–104
نویسندگان
, , , ,