کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2017541 1067802 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification and expression analysis of two small heat shock protein cDNAs from developing seeds of biodiesel feedstock plant Jatropha curcas
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Identification and expression analysis of two small heat shock protein cDNAs from developing seeds of biodiesel feedstock plant Jatropha curcas
چکیده انگلیسی

Plant small heat shock proteins (sHSPs) are known to be important for environmental stress tolerance and involved in various developmental processes. In this study, two full-length cDNAs encoding sHSPs, designated JcHSP-1 and JcHSP-2, were identified and characterized from developing seeds of a promising biodiesel feedstock plant Jatropha curcas by expressed sequence tag (EST) sequencing of embryo cDNA libraries and rapid amplification of cDNA ends (RACE). JcHSP-1 and JcHSP-2 contained open-reading frames encoding sHSPs of 219 and 157 amino acids, with predicted molecular weights of 24.42 kDa and 18.02 kDa, respectively. Sequence alignment indicated that both JcHSP-1 and JcHSP-2 shared high similarity with other plant sHSPs. Real-time quantitative RT-PCR analysis showed that the transcriptional level of both JcHSP-1 and JcHSP-2 increased along with natural dehydration process during seed development. A sharp increase of JcHSP-2 transcripts occurred in response to water content dropping from 42% in mature seeds to 12% in dry seeds. Western blot analysis revealed that the accumulation profile of two cross-reacting proteins, whose molecular weight corresponding to the calculated size of JcHSP-1 and JcHSP-2, respectively, was well consistent with the mRNA expression pattern of JcHSP-1 and JcHSP-2 in jatropha seeds during maturation and natural dehydration. These results indicated that both JcHSPs might play an important role in cell protection and seed development during maturation of J. curcas seeds.


► Two cDNAs of small heat shock proteins (sHSPs) were cloned from Jatropha curcas.
► Transcriptional level of both JcHSP-1 and JcHSP-2 increased during seed development.
► Sharp increase of JcHSP-2 transcripts occurred in dry seeds compared to mature seeds.
► Accumulation profile of JcHSP-1 and JcHSP-2 proteins was consistent with their mRNA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 181, Issue 6, December 2011, Pages 632–637
نویسندگان
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