Differential gene expression in different types of Hevea brasiliensis roots

Three types of roots (taproots, first order laterals and second order laterals) were functionally characterized on 7-month-old in vitro plantlets regenerated by somatic embryogenesis in Hevea brasiliensis. A histological analysis revealed different levels of differentiation depending on root diameter. A primary structure was found in first and second order lateral roots, while taproots displayed a secondary structure. The expression of 48 genes linked to some of the regulatory pathways acting in roots was compared in leaves, stems and the different types of roots by real-time RT-PCR. Thirteen genes were differentially expressed in the different organs studied in plants grown under control conditions. Nine additional other genes were differentially regulated between organs under water deficit conditions. In addition, 10 genes were significantly regulated in response to water deficit, including 8 regulated mainly in lateral roots types. Our results suggest that the regulation of gene expression in lateral roots is different than that in taproots, which have a main role in nutrient uptake and transport, respectively.

► First histological and gene expression analyses in Hevea brasiliensis roots.
► Description of three types of roots (taproots, first order lateral roots and second order lateral roots).
► First gene expression analysis in three types of root in woody species.
► Under a moderate water stress, relative accumulation of transcripts varied dramatically in lateral roots.
► This work suggests that most of the biological activities are taking place in fine lateral roots.