کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2017647 1067810 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A splice variant of Arabidopsis mitogen-activated protein kinase and its regulatory function in the MKK6–MPK13 pathway
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
A splice variant of Arabidopsis mitogen-activated protein kinase and its regulatory function in the MKK6–MPK13 pathway
چکیده انگلیسی

Alternative splicing (AS) generates multiple forms of proteins. A role for AS in the plant mitogen-activated protein kinase (MAPK) cascade has not been clarified. In this study, we analyzed expression of 20 Arabidopsis MAPK genes by RT-PCR and found five that generate splice variants. The MPK13 gene, with six exons and five introns, generates at least three splice variants, one in which complete splicing of five introns occurs (MPK13 Full), and ones in which the 4th and 5th introns are retained (MPK13 I4 and I5). Translation products of the splice variants MPK13 Full and I4, were found in Arabidopsis tissues by Western blot. Yeast two-hybrid analysis and protein kinase assays of recombinant proteins showed that neither I4 nor I5 interacted with upstream MAPKKs, and neither had protein kinase activity. However, MPK13 I4 protein enhanced the MKK6-dependent activation of MPK13 Full, indicating the possibility of an additional mechanism to regulate the MAPK cascade by AS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 178, Issue 3, March 2010, Pages 245–250
نویسندگان
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