Recent advances on the regulation of anthocyanin synthesis in reproductive organs

Anthocyanins represent the major red, purple, violet and blue pigments in many flowers and fruits. They attract pollinators and seed dispersers and defend plants against abiotic and biotic stresses. Anthocyanins are produced by a specific branch of the flavonoid pathway, which is differently regulated in monocot and dicot species. In the monocot maize, the anthocyanin biosynthesis genes are activated as a single unit by a ternary complex of MYB-bHLH-WD40 transcription factors (MBW complex). In the dicot Arabidopsis, anthocyanin biosynthesis genes can be divided in two subgroups: early biosynthesis genes (EBGs) are activated by co-activator independent R2R3-MYB transcription factors, whereas late biosynthesis genes (LBGs) require an MBW complex. In addition to this, a complex regulatory network of positive and negative feedback mechanisms controlling anthocyanin synthesis in Arabidopsis has been described. Recent studies have broadened our understanding of the regulation of anthocyanin synthesis in flowers and fruits, indicating that a regulatory system based on the cooperation of MYB, bHLH and WD40 proteins that control floral and fruit pigmentation is common to many dicot species.

► Anthocyanins are the final products of a specific branch of the flavonoid pathway.
► In maize, anthocyanin biosynthetic genes are activated by an MBW complex.
► In Arabidopsis, EBGs are activated by MYB TFs and LBGs by an MBW complex.
► In Arabidopsis, positive/negative feedback mechanisms control anthocyanin synthesis.
► Flower/fruit pigmentation is controlled by an MBW complex in many dicot species.