کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2018056 | 1067832 | 2007 | 10 صفحه PDF | دانلود رایگان |
A TuMV-NIb gene fragment was amplified from the total RNA of Chinese cabbage leaves infected with TuMV-C4 by RT-PCR. This fragment was cloned into the marker-free plant binary vector pROK (a derivative of pROK2) in the antisense orientation and transformed into Chinese cabbage [Brassica campestris ssp. pekinensis (Lour) Olsson] by the Agrobacterium tumefaciens strain LBA4404 using infiltration gene-transformation system. Forty-three transgenic T0 plants were obtained. PCR, ELISA and Southern blot analysis showed that the TuMV-NIb gene had integrated into the genome of Chinese cabbage and the heredity of this gene in some lines followed the 3:1 ratio. Both T0 and T1 transgenic plants showed high levels of resistance to TuMV when artificially inoculated with TuMV. RT-PCR analysis of T0 transgenic Chinese cabbage revealed the integrated TuMV-NIb gene can be expressed normally. The infiltration gene-transformation system had proved effective in transformation of Chinese cabbage.
Journal: Plant Science - Volume 172, Issue 5, May 2007, Pages 920–929