کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2018367 | 1067847 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation and analysis of differentially expressed genes in dominant genic male sterility (DGMS) Brassica napus L. using subtractive PCR and cDNA microarray
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Isolation and analysis of differentially expressed genes in dominant genic male sterility (DGMS) Brassica napus L. using subtractive PCR and cDNA microarray Isolation and analysis of differentially expressed genes in dominant genic male sterility (DGMS) Brassica napus L. using subtractive PCR and cDNA microarray](/preview/png/2018367.png)
چکیده انگلیسی
Dominant genic male sterility (DGMS) is an important approach to utilize the heterosis of Brassica napus, but the molecular mechanism of DGMS is not well understood. As an initial step towards understanding this event, some pilot studies were performed. Using suppression subtractive hybridization (SSH) and cDNA microarray, about 1200 significantly differentially expressed clones were isolated between the fertile and sterile plants of the homozygous DGMS two-type line, Rs1046AB. Northern blot further demonstrated the credibility of the microarray data. Subsequently, about 400 clones were selected for sequencing and they represented 216 unigenes (212 were up-regulated in fertile buds, and the other 4 were up-regulated in sterile ones). Of the 212 up-regulated genes in forward-subtracted library, 178 homologous sequences could be divided into 17 groups excluding those that encode the unclassified proteins; and the other 34 genes had no homology in the databases at the National Center for Biotechnical Information (NCBI). Furthermore, some important pathways related to male gametogenesis were identified using the program of KEGG Orthology (KO)-Based Annotation System (KOBAS), such as nitrogen metabolism, nitrobenzene degradation and starch and sucrose metabolism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 172, Issue 2, February 2007, Pages 204-211
Journal: Plant Science - Volume 172, Issue 2, February 2007, Pages 204-211
نویسندگان
Wu Jianyong, Shen Junru, Mao Xizeng, Liu Kede, Wei Liping, Liu Pingwu, Yang Guangsheng,