کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2018618 1067863 2006 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins
چکیده انگلیسی

Genetically transformed lines of the elite indica rice cultivars, ADT38, ASD16, IR50 and Pusa Basmati1, constitutively over-expressing rice tlp encoding a thaumatin-like protein, have been evolved for management of rice sheath blight disease. The incorporation of tlp transgene in the genomes of T0 lines was ascertained by polymerase chain reaction and confirmed by Southern hybridization analyses. Expression of tlp in the putative transformants was confirmed by western blotting analysis. Stable inheritance of the transgene expression was studied up to T2 generation by western blotting analysis. The putative transformants and their progenies expressing tlp showed enhanced resistance against the sheath blight pathogen, Rhizoctonia solani, when compared to the non-transformed plants. The use of rice chi11, encoding a chitinase, as a co-transgene along with tlp produced a tlp–chi11 co-transformant that showed enhanced resistance against R. solani than the ones that express tlp or chi11 transgene alone. In addition to sheath blight resistance, the tlp or chi11 transgenic lines displayed significant levels of protection against the rice sheath rot pathogen, Sarocladium oryzae.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 170, Issue 2, February 2006, Pages 203–215
نویسندگان
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