PGE2 through the EP4 receptor controls smooth muscle gene expression patterns in the ductus arteriosus critical for remodeling at birth

► Mice deficient in EP4 expression in smooth muscle (SMC) and endothelial cells. ► EP4 expression only in SMC is required for ductus arteriosus (DA) closure. ► Genome wide gene expression analysis of WT and EP4−/− DA. ► Identification of PGE2/EP4 dependent gene expression in the DA.