Tumor necrosis factor-alpha induces renal cyclooxygenase-2 expression in response to hypercalcemia

► The effect of TNF on COX-2 expression was determined in the renal OM. ► An elevation in urinary TNF was seen in WT mice given a vitamin D analog (DHT). ► An increase in urinary PGE2 was observed in WT mice treated with DHT. ► COX-2 expression in the OM was elevated in WT but not TNF−/− mice given DHT. ► We demonstrated that during hypercalcemia COX-2 expression in the renal OM is TNF-dependent.