کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
20198 43162 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of kojic acid-grafted-chitosan oligosaccharides as a novel antibacterial agent on cell membrane of gram-positive and gram-negative bacteria
ترجمه فارسی عنوان
اثر الیگوساکارید های اسید گاو-کتوکسیتوزان به عنوان یک عامل ضد باکتری جدید بر روی غشای سلولی باکتری گرم مثبت و گرم منفی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• The modes of action of COS/KA on Gram-positive and Gram-negative bacteria were examined.
• COS/KA increased the membrane permeability by adhering to cell membranes.
• COS/KA resulted in leakage of cell inclusions (enzymes, DNA, proteins and ions).
• Gram-positive bacteria were more sensitive than Gram-negative bacteria to COS/KA.
• COS/KA has potential in food industry and biomedical sciences.

Our work here, for the first time, reported the antibacterial activity of kojic acid-grafted-chitosan oligosaccharides (COS/KA) against three gram-positive and three gram-negative bacteria. Integrity of cell membrane, outer membrane (OM) and inner membrane (IM) permeabilization assay, alkaline phosphatase (ALP) and glucose-6-phosphate dehydrogenase (G6PDH) assay, and SDS-PAGE assay techniques were used to investigate the interactions between COS/KA and bacterial membranes. The antibacterial activity of COS/KA was higher than those of unmodified COS. The electric conductivity of bacteria suspensions increased, followed by increasing of the units of average release for ALP and G6PDH. COS/KA can also rapidly increase the 1-N-phenylanphthylamine (NPN) uptake and the release of β-galactosidase via increasing the permeability of OM and IM in Escherichia coli. SDS-PAGE indicated the content of cellular soluble proteins decreased significantly in COS/KA-treated bacteria. Hence, COS/KA has potential in food industry and biomedical sciences.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 3, September 2015, Pages 335–339
نویسندگان
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