کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2020076 | 1069096 | 2007 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Relaxing effects of 5-oxo-ETE on human bronchi involve BKCa channel activation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The present study investigated the ability of 5-oxo-EicosaTetraEnoic acid (5-oxo-ETE) for modulating airway smooth muscle (ASM) tone in human bronchi. 5-Oxo-ETE induced a concentration-dependent relaxing effect on human bronchi pre-contracted with methacholine (MCh) and arachidonic acid (AA). This relaxing response was highly sensitive to Iberiotoxin (IbTx), a large conducting Ca2+-activated K+ channel (BKCa) inhibitor. Furthermore, microelectrode measurements revealed that 5-oxo-ETE (0.1-10 μM) hyperpolarizes the membrane potential of human bronchial ASM cells. These hyperpolarizing effects were also inhibited in the presence of 10 nM IbTx. Lastly, 5-oxo-ETE was shown to directly activate reconstituted BKCa channels derived from human airway smooth muscles. In summary, the 5-oxo-ETE eicosanoid activates a specific K+ conductance, involved in membrane hyperpolarization, which in turn reduces Ca2+ entry and facilitates relaxation of smooth muscle cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Prostaglandins & Other Lipid Mediators - Volume 83, Issue 4, June 2007, Pages 311-319
Journal: Prostaglandins & Other Lipid Mediators - Volume 83, Issue 4, June 2007, Pages 311-319
نویسندگان
Caroline Morin, Marco Sirois, Vincent Ãchave, Marcio M. Gomes, Eric Rousseau,