کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2020124 1542254 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
17-β-Estradiol upregulates COX-2 in the rat oviduct
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
17-β-Estradiol upregulates COX-2 in the rat oviduct
چکیده انگلیسی

We investigated the regulation of cyclooxygenase-2 (COX-2) by 17-β-estradiol (E2) in the rat oviduct. We observed that COX-2 is expressed mainly in proestrous and estrous stages, periods under estrogenic influence. While exogenous administration of E2 (1 μg/rat) significantly increased COX-2 protein levels, progesterone did not modify it. COX-2 was mainly localized on oviductal epithelial cells from estrogenized rat. Induction of COX-2 expression by E2 was partially reverted by tamoxifen (1 mg/rat), an E2 receptor antagonist. Estradiol treatment also increased prostaglandins (PGs) synthesis: 6-keto-PGF1α (40%), a stable metabolite of prostacyclin (PGI2), PGF2α (40%) and PGE2 (50%). Tamoxifen completely suppressed this enhancement. In order to discriminate which isoform of COX was implicated in the stimulatory effect of E2 on PGs synthesis, oviducts were preincubated with meloxicam (Melo: 10−9 M) or NS-398 (10−7 M), two selective COX-2 inhibitors. Both Melo and NS-398 abolished the increase of PGs synthesis stimulated by E2. All together, these data indicate that E2 could upregulate COX-2 expression and activity in the rat oviduct and that the stimulatory effect of E2 may be receptor-mediated.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Prostaglandins & Other Lipid Mediators - Volume 80, Issues 3–4, September 2006, Pages 155–164
نویسندگان
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