Developments in purification methods for obtaining and evaluation of collagen derived endogenous angioinhibitors

• Developments in methods for obtaining collagen derived endogenous angioinhibitors.
• A comprehensive review discussed different purification methods.
• Sequential developmental methodology from history.
• Various modified methods to improve higher yields of biologically active collagen proteins.
• This review would be of broad interest to many protein biologists for purification methods.

Collagen constitutes one of the vital components of the basement membrane scaffolds. Non-collagenous domains (NC1) derived from collagens exhibit potent anti-angiogenic properties, thus attaining significance in regulation of angiogenesis promoted diseases. Individual NC1 domains essential for anti-angiogenic evaluations are generally obtained through purification of individual non-collagenous domains, which have undergone steady developments for enhancing the yields, purpose of biological evaluations and solubility based on the nature of different NC1 domains. This review focuses on the method developments in obtaining biologically active NC1 domains and for specific evaluations in different scenarios.