Expression and purification of an immunogenic dengue virus epitope using a synthetic consensus sequence of envelope domain III and Saccharomyces cerevisiae

► We describe an expression and secretion of dengue virus epitope using baker's yeast. ► We describe a simple and efficient purification of yeast-expressed target protein. ► We describe the yeast-expressed scEDIII can elicit tetravalent antibodies. ► We describe a balanced immune response by the yeast-expressed target protein.