Soluble expression and purification of tumor suppressor WT1 and its zinc finger domain

► Expression of full-length, active WT1 in Escherichia coli. ► Identification of proteolytically labile sites in WT1 zinc finger domains. ► Binding of −KTS and +KTS zinc finger isoforms of authentic PDGFA promoter. ► Interaction of two molecules of full-lenght WT1 in binding PDGFA promoter.