Engineering of an elastic scaffolding polyprotein based on an SH3-binding intrinsically disordered titin PEVK module

► Successfully engineered titin PEVK polyproteins for nanomechanical measurements. ► Investigated systematically expression hosts for proteins with tandem repeats. ► Expressed and purified gram quantities of secreted polyproteins in a yeast system. ► Facilitated studies of the role of force in titin's function.