Enhanced purification and characterization of the PfeIF4A (PfH45) helicase from Plasmodium Falciparum using a codon-optimised clone

► Cloning, growth and purification of soluble Plasmodium falciparum eIF4A. ► PfeIF4A is a bipolar helicase and has a bias for 3′-5′ unwinding activity. ► Purified enzyme produced in yields greater than previously described and comparable in activity to previous sources.