Production of N-acetylgalactosaminyl-transferase 2 (GalNAc-T2) fused with secretory signal Igκ in insect cells

► Murine Igκ secretory signal drives secretion of fusion GalNAc-T2 in insect system. ► Secreted soluble human GalNAc-T2 was enzymatically active. ► The yield in Sf9 cells was 2.5 mg of GalNAc-T2 protein per 1 L culture. ► Recombinant GalNAc-T2 attaches GalNAc to all possible O-glycosylation sites in IgA1.