کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2020913 1069216 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae
چکیده انگلیسی

N-acetyl serotonin methyl transferase (ASMT) is the last enzyme in the melatonin synthesis pathway. Evidence linking autism-related disorders with disorders of melatonin metabolism, and, more specifically, with mutations of the gene encoding ASMT, prompted us to investigate the properties and localization of this enzyme. As a first step, we undertook to overproduce the protein in a recombinant host. Early attempts to produce ASMT in recombinant Escherichia coli yielded only insoluble and heavily degraded material. However, recombinant ASMT (rASMT) could be produced in soluble, active form and purified in milligram amounts when the gene was cloned and expressed in Leishmania tarentolae.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 75, Issue 1, January 2011, Pages 114–118
نویسندگان
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