کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2020936 1069218 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Appraisal of translocation pathways for displaying ankyrin repeat protein on phage particles
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Appraisal of translocation pathways for displaying ankyrin repeat protein on phage particles
چکیده انگلیسی
Depending on the molecular properties of the proteins of interest (POI), the rate of success in displaying proteins on phage particles is unpredictable. Formation of polypeptide tertiary structure in the cytoplasm occasionally results in low level display on viral particles. Here we assessed the influence of different leader peptides on the display of a premature cytoplasmic folding protein, ankyrin repeat protein (ARP), via the minor coat protein pIII. These peptides include the Sec, SRP and Tat pathways. The results demonstrated that the Sec and SRP pathways were capable of displaying the protein on the viral particle, whereas the Tat pathway failed to do so. Interestingly, the Tat pathway efficiently directed ARP through its translocon without fusing with pIII. Furthermore, the soluble form of ARP was detected in Escherichia coli periplasm.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 74, Issue 2, December 2010, Pages 156-161
نویسندگان
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