کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2020999 1542351 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inducible expression of chimpanzee prion protein (PrP) in murine PrP knock-out cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Inducible expression of chimpanzee prion protein (PrP) in murine PrP knock-out cells
چکیده انگلیسی

In transmissible spongiform encephalopathy (TSE) pathogenesis the cellular prion protein (PrPC) is converted into its pathogenic PrPSc isoform. Prion protein gene (Prnp) deficient mice (PrP0/0) are resistant to PrPSc infection, but following reconstitution of Prnp they regain their susceptibility to infection. Therefore, it is challenging to simulate this natural situation in a cell culture model. We have previously reported the inducible stable expression of a human PrPC in murine 3T3 cells. In this study, we used murine PrP0/0 cells stably expressing exemplarily the chimpanzee Prnp under the control of inducible tetracycline (Tet) system. The Prnp was integrated using a lentiviral vector. Its expression in the engineered PrP0/0Chimp1/Tet-Off cell line was analyzed by Western blot (Wb) and fluorescence activated cell sorting (FACS) analyses. PrPC was partially purified by using immobilized metal affinity chromatography (IMAC). Compared to all the other cell systems which possess an endogenous PrPC expression, here described cell line contains only an overexpressing species specific PrPC expression which is tightly regulated and can be turned-off at any time without showing any endogenous host PrPC expression. Consequently, a contamination of the isolated PrPC is impossible. This cell line potentially offers a new tool for simulation of mice bioassays widely used in TSE infection studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 70, Issue 2, April 2010, Pages 129–136
نویسندگان
, , , , ,