کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2021063 1069225 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Large-scale production of a disulfide-stabilized constitutively active mutant opsin
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Large-scale production of a disulfide-stabilized constitutively active mutant opsin
چکیده انگلیسی
Previous studies of constitutively activated mutants of opsin in the absence of chromophore were carried out in crude cell membranes because such mutants could not be recovered in a detergent-solubilized form in the active state. We employed a strategy in which a stabilizing disulfide bond allowed for successful purification of a constitutively activated mutant opsin, N2C/E113Q/M257Y/D282C, solubilized in nonionic detergent from mammalian cell culture. The purified mutant opsin is able to activate transducin to a higher degree than opsin and may prove useful for future structural studies of the active state of GPCRs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 75, Issue 2, February 2011, Pages 236-241
نویسندگان
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