کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2021138 | 1069230 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression, purification and characterization of the acyl carrier protein phosphodiesterase from Pseudomonas Aeruginosa
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Expression, purification and characterization of the acyl carrier protein phosphodiesterase from Pseudomonas Aeruginosa Expression, purification and characterization of the acyl carrier protein phosphodiesterase from Pseudomonas Aeruginosa](/preview/png/2021138.png)
چکیده انگلیسی
Acyl carrier protein phosphodiesterases (AcpH) are the only enzymes known to remove the 4â²-phosphopantetheinyl moiety from holo acyl carrier proteins (ACP), which are a large family of proteins essential for the biosynthesis of lipid and other cellular metabolites. Here we report that the AcpH (paAcpH) from Pseudomonas aeruginosa can be overexpressed in Escherichia coli as a soluble and stable protein after optimization of the expression and purification conditions. This marks an improvement from the aggregation-prone E. coli AcpH that could only be obtained by refolding the polypeptide obtained from the inclusion body. With the soluble recombinant protein, we found that PaAcpH exhibits preferred substrate specificity towards the ACPs from the fatty acid synthesis pathway among eight carrier proteins. We further showed that PaAcpH hydrolyzes and releases the 4â²-phosphopantetheinyl group-linked products from a multidomain polyketide synthase, demonstrating that the enzyme is fully capable of hydrolyzing acylated ACP substrates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 71, Issue 2, June 2010, Pages 132-138
Journal: Protein Expression and Purification - Volume 71, Issue 2, June 2010, Pages 132-138
نویسندگان
Elavazhagan Murugan, Rong Kong, Huihua Sun, Feng Rao, Zhao-Xun Liang,