کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2021144 | 1069230 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Establishment of a simple and rapid method to screen for strong promoters in Bacillus subtilis
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Establishment of a simple and rapid method to screen for strong promoters in Bacillus subtilis Establishment of a simple and rapid method to screen for strong promoters in Bacillus subtilis](/preview/png/2021144.png)
چکیده انگلیسی
Using published plasmid vectors containing the bgaB gene encoding a heat-stable β-galactosidase, we have been unable to fuse strong promoters to this reporter gene. In addition, we could not analyze the promoter strength by a plate assay. Therefore, we constructed an Escherichia coli–Bacillus subtilis shuttle vector to allow the cloning of strong promoters and their rapid analysis in B. subtilis by plating on X-Gal plates in the presence of the inducer IPTG. We show that the blue color of the colonies reflects the strength of the promoters, which was verified by measuring the β-galactosidase activities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 71, Issue 2, June 2010, Pages 174–178
Journal: Protein Expression and Purification - Volume 71, Issue 2, June 2010, Pages 174–178
نویسندگان
Trang Thi Phuong Phan, Hoang Duc Nguyen, Wolfgang Schumann,