کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2021185 1069234 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of location of the His-tag on the production of soluble and functional Buthus martensii Karsch insect toxin
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Effect of location of the His-tag on the production of soluble and functional Buthus martensii Karsch insect toxin
چکیده انگلیسی

The low yield and poor folding efficiency in vivo of soluble and active recombinant cysteine-rich proteins expressed in Escherichia coli are a major challenge for large-scale protein production and purification. Expression vectors containing Buthus martensii Karsch insect toxin (BmK IT) fused to the C terminus of the intein Ssp DnaB were constructed in an attempt to overcome this problem. Following purification and intein self-cleavage, the fusion protein His6-intein-IT produced insoluble BmK IT, while intein-IT-His6 generated soluble and properly folded BmK IT. This result indicated that the positioning of the His6 tag has a key role in the production of soluble and functional BmK IT.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 59, Issue 1, May 2008, Pages 103–109
نویسندگان
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